邓立新;钟玲;温春虹;苏军凯;李莉;胡志钰;张鸣青;

• 1:厦门大学附属东南医院解放军第175医院
• 2:厦门大学生命科学学院

摘要(Abstract)：

测定健康小鼠进食绣球菌对腹腔巨噬细胞分泌肿瘤坏死因子α(TNF-α)的影响.SPF级Balb/C健康小鼠分组进食普通饲料、分别含绣球菌饲料、干品、浸汁饮水或其添加物组合、分别含酵母β-葡聚糖饲料、浸汁饮水或其添加物组合;进食一定时间后,分离腹腔巨噬细胞,使用含质量分数10%胎牛血清的RPMI1640培养液培养,TNF-α试剂盒检测上清液中的TNF-α质量浓度.结果表明,强化进食绣球菌组合(绣球菌饲料+干品+浸汁)的小鼠与对照组或酵母β-葡聚糖组合(酵母β-葡聚糖饲料+浸汁)小鼠相比,体质量不升反降;分离的腹腔巨噬细胞培养6 d,酶联免疫吸附测定技术检测上清液TNF-α,其质量浓度远高于其它两组.研究提示,强化绣球菌进食组合能够提高健康小鼠免疫力,并可能有利于控制体质量.

关键词(KeyWords)： 巨噬细胞;肿瘤坏死因子α;绣球菌;酶联免疫吸附测定技术;酵母β-葡聚糖

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金资助项目(2013J01394);; 吴阶平医学基金会临床科研项目(320.6750.15231);; 空军军医大学国重开放课题(CBSKL201729);; 福建省中医药大学校管课题(XB2015077)

作者(Authors): 邓立新;钟玲;温春虹;苏军凯;李莉;胡志钰;张鸣青;

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