健康小鼠进食绣球菌对腹腔巨噬细胞分泌肿瘤坏死因子α的影响Orally Administered Sparassis crispa Affects TNF-α Secreting by Peritoneal Macrophages from Healthy Mice
邓立新;钟玲;温春虹;苏军凯;李莉;胡志钰;张鸣青;
摘要(Abstract):
测定健康小鼠进食绣球菌对腹腔巨噬细胞分泌肿瘤坏死因子α(TNF-α)的影响.SPF级Balb/C健康小鼠分组进食普通饲料、分别含绣球菌饲料、干品、浸汁饮水或其添加物组合、分别含酵母β-葡聚糖饲料、浸汁饮水或其添加物组合;进食一定时间后,分离腹腔巨噬细胞,使用含质量分数10%胎牛血清的RPMI1640培养液培养,TNF-α试剂盒检测上清液中的TNF-α质量浓度.结果表明,强化进食绣球菌组合(绣球菌饲料+干品+浸汁)的小鼠与对照组或酵母β-葡聚糖组合(酵母β-葡聚糖饲料+浸汁)小鼠相比,体质量不升反降;分离的腹腔巨噬细胞培养6 d,酶联免疫吸附测定技术检测上清液TNF-α,其质量浓度远高于其它两组.研究提示,强化绣球菌进食组合能够提高健康小鼠免疫力,并可能有利于控制体质量.
关键词(KeyWords): 巨噬细胞;肿瘤坏死因子α;绣球菌;酶联免疫吸附测定技术;酵母β-葡聚糖
基金项目(Foundation): 福建省自然科学基金资助项目(2013J01394);; 吴阶平医学基金会临床科研项目(320.6750.15231);; 空军军医大学国重开放课题(CBSKL201729);; 福建省中医药大学校管课题(XB2015077)
作者(Authors): 邓立新;钟玲;温春虹;苏军凯;李莉;胡志钰;张鸣青;
参考文献(References):
- [1]CHEN Y,DONG L,WENG D,et al.1,3-beta-Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti-inflammatory cytokines in vitro[J].Molecular Medicine Reports,2013,8(2):708-712.
- [2]MANTOVANI M S,BELLINI M F,ANGELI J P,et al.Beta-Glucans in promoting health:prevention against mutation and cancer[J].Mutat Res,2008,658(3):154-161.
- [3]CHEN J,SEVIOUR R.Medicinal importance of fungal beta-(1→3),(1→6)-glucans[J].Mycol Res,2007,111(6):635-652.
- [4]LINDEQUIST U,NIEDERMEYER T H J,JULICH W D.The pharmacological potential of mushrooms[J].EvidenceBased Complementary and Alternative Medicine,2005,2(3):285-299.
- [5]CHEN J,HUANG X F.The effects of diets enriched in beta-glucans on blood lipoprotein concentrations[J].J Clin Lipidol,2009,3(3):154-158.
- [6]CHEN J,RAYMOND K.Beta-glucans in the treatment of diabetes and associated cardiovascular risks[J].Vasc Health Risk Manag,2008,4(6):1265-1272.
- [7]CHAN G C-F,CHAN W K,SZE D M-Y.The effects of beta-glucan on human immune and cancer cells[J].Journal of Hematology&Oncology,2009,2(25):1-11.
- [8]BOHN J A,BEMILLER J N.(1->3)-beta-D-glucans as biological response modifiers:a review of structure-functional activity relationships[J].Carbohydr Polym,1995,28(1):3-14.
- [9]ALEEM E.β-Glucans and their applications in cancer therapy:focus on human studies[J].Anticancer Agents Med Chem,2013,13(5):709-719.
- [10]BORCHERS A T,STERN J S,HACKMAN R M,et al.Mushrooms,tumors,and immunity[J].Proc Soc Exp Biol Med,1999,221(4):281-293.
- [11]BASHIR K,CHOI J S.Clinical and physiological perspectives ofβ-Glucans:the past,present,and future[J].International Journal of Molecular Sciences,2017,18(9):1906-1954.
- [12]OHNO N,MIURA N N,NAKAJIMA M,et al.Antitumor 1,3-beta-glucan from cultured fruit body of Sparassis crispa[J].Biological&Pharmaceutical Bulletin,2000,23(7):866-872.
- [13]HARADA T,MIURA N N,ADACHI Y,et al.IFN-gamma induction by SCG,1,3-beta-D-glucan from Sparassis crispa,in DBA/2 mice in vitro[J].Journal of Interferon and Cytokine Research,2002,22(12):1227-1239.
- [14]YAMAMOTO K,KIMURA T,SUGITACHI A,et al.Anti-angiogenic and anti-metastatic effects of beta-1,3-D-glucan purified from hanabiratake,Sparassis crispa[J].Biological&Pharmaceutical Bulletin,2009,32(2):259-263.
- [15]HARADA T,MIURA N,ADACHI Y,et al.Effect of SCG,1,3-beta-D-glucan from Sparassis crispa on the hematopoietic response in cyclophosphamide induced leukopenic mice[J].Biological&Pharmaceutical Bulletin,2002,25(7):931-939.
- [16]YAMAMOTO K AND KIMURA T.Orally and topically administered Sparassis crispa(Hanabiratake)improved healing of skin wounds in mice with streptozotocin-induced diabetes[J].Bioscience Biotechnology and Biochemistry,2013,77(6):1303-1305.
- [17]KWON A H,QIU Z Y,HASHIMOTO M,et al.Effects of medicinal mushroom(Sparassis crispa)on wound healing in streptozotocin-induced diabetic rats[J].American Journal of Surgery,2009,197(4):503-509.
- [18]LEE S Y,LEE Y G,BYEON S E,et al.Mitogen activated protein kinases are prime signalling enzymes in nitric oxide production induced by soluble beta-glucan from Sparassis crispa[J].Archives of Pharmacal Research,2010,33(11):1753-1760.
- [19]YOSHITOMI H,IWAOKA E,KUBO M,et al.Beneficial effect of Sparassis crispa on stroke through activation of Akt/e NOS pathway in brain of SHRSP[J].Journal of Natural Medicines,2011,65(1):135-141.
- [20]BERNER M D,SURA M E,ALVES B N,et al.IFN-gamma primes macrophages for enhanced TNF-alpha expression in response to stimulatory and non-stimulatory amounts of microparticulate beta-glucan[J].Immunol Lett,2005,98(1):115-122.