马尾松银松素合酶基因转化双子叶植物表达载体的构建及鉴定Construction and Characterization of Dicotyledon Expression Vector Containing Pinosylvin Synthase Gene from Pinus massoniana
王冰梅;张积森;黄庆煌;叶冰莹;陈如凯;陈由强;
摘要(Abstract):
以马尾松为材料,在获得马尾松银松素合酶基因的基础上,构建了该基因转化双子叶植物的表达载体,该表达载体含有35S启动子和NOS终止子,能启动基因在双子叶植物中高效地表达.设计并合成分别含有Bgl和BstE酶切位点的上下游引物,通过PCR反应扩增出含有该酶切位点的银松素合酶基因cDNA全序列,酶切后连接到pCAMBIA1301植物表达载体上.经PCR鉴定、酶切分析及DNA测序证实cDNA片段大小、序列以及读码框的正确性.最后通过电击将表达载体转化农杆菌LBA4404,为进一步研究银松素合酶基因的功能奠定基础.
关键词(KeyWords): 银松素合酶;35S;双子叶植物;表达载体;构建
基金项目(Foundation): 国家自然科学基金资助项目(30371177);; 福建省教育厅基金资助项目(JA07045)
作者(Authors): 王冰梅;张积森;黄庆煌;叶冰莹;陈如凯;陈由强;
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