王瑜华;蒋宁城;徐朝贤;杨洪钦;谢树森;

• 1:医学光电科学与技术教育部重点实验室福建省光子技术重点实验室福建师范大学激光与光电子技术研究所

摘要(Abstract)：

给出原子力显微术(AFM)在活细胞表面超微结构成像和生物力学特性检测的技术方案,概述了AFM在肿瘤细胞生物物理特性研究的最新进展及其重要意义.

关键词(KeyWords)： 原子力显微术;肿瘤细胞;生物物理特性;超微结构;纳米药物

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973计划)资助项目(2015CB352006);; 国家自然科学基金重点资助项目(61335011);; 福建省教育厅项目(JB13002);; 福州市科技计划项目(2014S1372)

作者(Authors): 王瑜华;蒋宁城;徐朝贤;杨洪钦;谢树森;

参考文献(References)：

• [1]SIEGEL R,MA J,ZOU Z,et al.Cancer statistics,2014[J].CA:A Cancer Journal for Clinicians,2014,64(1):9-29.
• [2]ENGELBERTH S A,HEMPEL N,BERGKVIST M.Development of nanoscale approaches for ovarian cancer therapeutics and diagnostics[J].Crit Rev Oncog,2014,19(3/4):281-315.
• [3]LUO L,DONG L Y,YAN Q G et al.Research progress in applying proteomics technology to explore early diagnosis biomarkers of breast cancer,lung cancer and ovarian cancer[J].Asian Pac J Cancer Prev,2014,15(20):8529-8538.
• [4]WAN T S,MA E S.Molecular cytogenetics:an indispensable tool for cancer diagnosis[J].Chang Gung Med J,2012,35(2):96-110.
• [5]BARRETO J A,O’MALLEY W,KUBEIL M,et al.Nanomaterials:applications in cancer imaging and therapy[J].Advanced Materials,2011,23(12):H18-H40.
• [6]CHENG R,MENG F,DENG C,et al.Dual and multi-stimuli responsive polymeric nanoparticles for programmed sitespecific drug delivery[J].Biomaterials,2013,34(14):3647-3657.
• [7]GE Z,LIU S.Functional block copolymer assemblies responsive to tumor and intracellular microenvironments for site-specific drug delivery and enhanced imaging performance[J].Chemical Society Reviews,2013,42(17):7289-7325.
• [8]GIESSIBL F J.Atomic resolution of the silicon(111)-(7x7)surface by atomic force microscopy[J].Science,1995,267(5194):68-71.
• [9]GROSS L,MOHN F,MOLL N,et al.The chemical structure of a molecule resolved by atomic force microscopy[J].Science,2009,325(5944):1110-1114.
• [10]LIU S,WANG Y.Application of AFM in microbiology:a review[J].Scanning,2010,32(2):61-73.
• [11]HINTERDORFER P,DUFRêNE Y F.Detection and localization of single molecular recognition events using atomic force microscopy[J].Nature Methods,2006,3(5):347-355.
• [12]ALLISON D P,MORTENSEN N P,SULLIVAN C J,et al.Atomic force microscopy of biological samples[J].Wiley Interdisciplinary Reviews:Nanomedicine and Nanobiotechnology,2010,2(6):618-634.
• [13]LI K,ZHANG W,CHEN Y.Quantum dot binding to DNA:Single-molecule imaging with atomic force microscopy[J].Biotechnology Journal,2013,8(1):110-116.
• [14]ALBRECHT T R,QUATE C F.Atomic resolution imaging of a nonconductor by atomic force microscopy[J].Journal of Applied Physics,1987,62(7):2599-2602.
• [15]MEYER E.Atomic force microscopy[J].Progress in Surface Science,1992,41(1):3-49.
• [16]HANSMA P K,CLEVELAND J P,RADMACHER M,et al.Tapping mode atomic force microscopy in liquids[J].Applied Physics Letters,1994,64(13):1738-1740.
• [17]CAPPELLA B,DIETLER G.Force-distance curves by atomic force microscopy[J].Surface Science Reports,1999,34(1):1-104.
• [18]DUFRêNE Y F,EVANS E,ENGEL A,et al.Five challenges to bringing single-molecule force spectroscopy into living cells[J].Nature Methods,2011,8(2):123-127.
• [19]MüLLER D J,DUFRêNE Y F.Force nanoscopy of living cells[J].Current Biology,2011,21(6):R212-R216.
• [20]BUTT H J,CAPPELLA B,KAPPL M.Force measurements with the atomic force microscope:Technique,interpretation and applications[J].Surface Science Reports,2005,59(1):1-152.
• [21]LEE G Y H,LIM C T.Biomechanics approaches to studying human diseases[J].Trends in Biotechnology,2007,25(3):111-118.
• [22]CHEN J.Nanobiomechanics of living cells:a review[J].Interface Focus,2014,4(2):20130055.
• [23]LEKKA M,LAIDLER P,GIL D,et al.Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy[J].European Biophysics Journal,1999,28(4):312-316.
• [24]FARIA E C,Ma N,GAZI E,et al.Measurement of elastic properties of prostate cancer cells using AFM[J].Analyst,2008,133(11):1498-1500.
• [25]LI Q S,Lee G Y H,Ong C N,et al.AFM indentation study of breast cancer cells[J].Biochemical and Biophysical Research Communications,2008,374(4):609-613.
• [26]LYER S,GAIKWAD R M,SUBBA-RAO V,et al.Atomic force microscopy detects differences in the surface brush of normal and cancerous cells[J].Nature Nanotechnology,2009,4(6):389-393.
• [27]CROSS S E,JIN Y S,RAO J,et al.Nanomechanical analysis of cells from cancer patients[J].Nature Nanotechnology,2007,2(12):780-783.
• [28]CROSS S E,JIN Y S,TONDRE J,et al.AFM-based analysis of human metastatic cancer cells[J].Nanotechnology,2008,19:384003.
• [29]REBELO L M,DE SOUSA J S,MENDES FILHO J,et al.Comparison of the viscoelastic properties of cells from different kidney cancer phenotypes measured with atomic force microscopy[J].Nanotechnology,2013,24:055102.
• [30]XU Chaoxian,WANG Yuhua,JIANG Ningcheng,et al.Elasticity measurement of breast cancer cells by atomic force microscopy[J].Proc SPIE 9230,Twelfth International Conference on Photonics and Imaging in Biology and Medicine,2014,9230:92300Y.
• [31]SOKOLOV I.Atomic force microscopy in cancer cell research[J].Cancer Nanotechnology,2007,1:1-17.
• [32]SHI X,ZHANG X,XIA T,et al.Living cell study at the single-molecule and single-cell levels by atomic force microscopy[J].Nanomedicine,2012,7(10):1625-1637.
• [33]PILLET F,CHOPINET L,FORMOSA C,et al.Atomic Force Microscopy and pharmacology:From microbiology to cancerology[J].Biochimica et Biophysica Acta(BBA)-General Subjects,2014,1840(3):1028-1050.
• [34]MCNAMEE C E,ARMINI S,YAMAMOTO S,et al.Determination of the binding of non-cross-linked and cross-linked gels to living cells by atomic force microscopy[J].Langmuir,2009,25(12):6977-6984.
• [35]RAMACHANDRAN S,QUIST A P,KUMAR S,et al.Cisplatin nanoliposomes for cancer therapy:AFM and fluorescence imaging of cisplatin encapsulation,stability,cellular uptake,and toxicity[J].Langmuir,2006,22(19):8156-8162.
• [36]WANG J,WAN Z,LIU W,et al.Atomic force microscope study of tumor cell membranes following treatment with anticancer drugs[J].Biosensors and Bioelectronics,2009,25(4):721-727.
• [37]KIM K S,CHO C H,PARK E K,et al.AFM-detected apoptotic changes in morphology and biophysical property caused by paclitaxel in Ishikawa and He La cells[J].Plo S One,2012,7(1):e30066.