曾世涌;温真;何海滔;杨彩云;毛惠民;杨梅;

• 1:福建师范大学生命科学学院

摘要(Abstract)：

根据NCBI上已公布的枯草芽孢杆菌BS168菌株的β-1,4-glucanase(eglS)基因以及苏云金芽孢杆菌的aiiA基因序列,设计引物并扩增得到eglS基因的启动子、信号肽序列和aiiA基因.构建重组表达载体Ps4-eaiiA,转化枯草芽孢杆菌BS168,得到枯草芽孢杆菌工程菌BS168/Ps4-eaiiA.该工程菌诱导发酵后经SDS-PAGE和Western blotting分析表明:aiiA基因实现了在枯草芽孢杆菌中的表达.对胡萝卜软腐欧文氏菌进行马铃薯的抗病性实验,结果表明aiiA基因在枯草芽孢杆菌中表达的AiiA蛋白对胡萝卜欧文氏杆菌表现出一定的抗病性.

关键词(KeyWords)： 苏云金芽胞杆菌;AiiA蛋白;枯草芽孢杆菌表达

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金资助项目(2012J01123);; 福建省教育厅资助项目(JK2012009);; 福建省大学生创新创业训练计划资助项目(sjcxcy-2012028)

作者(Authors): 曾世涌;温真;何海滔;杨彩云;毛惠民;杨梅;

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