重金属离子对栅藻生长代谢的影响Effect of Heavy Metals on Growth and Metabolism in Scenedesmus sp.
王珊珊;王明兹;李敏;陈必链;
摘要(Abstract):
研究了Pb、Zn、Cu、Cd、Mn、Cr、Ni等7种重金属离子对栅藻生长代谢的影响.定时测定重金属处理后藻的生长情况、叶绿素a和粗油脂含量.结果表明,栅藻对Pb的耐受性最强,耐受能力可达20 mg·L-1,Zn、Mn、Ni其次,对Cu、Cd、Cr比较敏感,耐受性仅为1 mg·L-1.当重金属离子质量浓度在1 mg·L-1以下,Pb、Zn促进栅藻生长,同时提高其叶绿素a含量.Pb质量浓度为0.5 mg·L-1时,生物量最高,达0.642 mg·L-1,随着质量浓度提高,粗油脂含量增加,脂肪酸主要组分为棕榈酸甲酯、油酸甲酯、亚油酸甲酯、亚麻酸甲酯,并且随着胁迫质量浓度的增加,脂肪酸含量明显增加.超过一定质量浓度后,重金属离子抑制栅藻的生长,并导致生物量下降,Zn质量浓度高于5 mg·L-1时,栅藻叶绿素a含量降至1 mg·L-1以下.
关键词(KeyWords): 重金属;栅藻;耐受性;生长代谢
基金项目(Foundation): 福建省发展与改革委员会资助项目(闽发改高技[2011]1598);; 福建省环境保护厅资助项目(FJHK200912)
作者(Authors): 王珊珊;王明兹;李敏;陈必链;
参考文献(References):
- [1]Pahlavanzadeha H,Keshtkarb A R,Safdarib J,et al.Biosorption of nickel(Ⅱ)from aqueous solution by brown algae:E-quilibrium,dynamic and thermodynamic studies[J].Journal of Hazardous Materials,2010,175(1/3):304-310.
- [2]王坎,李敦海,刘永定,等.水华蓝藻生物质对Cu和Cr金属离子的生物吸附[J].水生生物学报,2011,35(6):1056-1059.
- [3]Malgorzata R,Andrzej K,Maria W.Sorption properties of algae Spirogyra sp.and their use for determination of heavy met-al ions concentrations in surface water[J].Bioelectrochemistry,2010,80(1):81-86.
- [4]Anastasakis K,Ross A B,Jones J M.Pyrolysis behaviour of the main carbohydrates of brown macroalgae[J].Fuel,2011,90(2):598-607.
- [5]沈建忠,陆德祥,张跃群,等.Cr3+、Zn2+对亚心型大扁藻生长的影响[J].安徽农业科学,2009,37(13):5840-5842.
- [6]Mehta S K,Gaur J P.Use of algae for removing heavy metal ions from wastewat:progress and prospects[J].Critical Re-views in Biotechnology,2005,25(3):113-152.
- [7]Piovar J,Stavrou E,Kadukova J,et al.Influence of long-term exposure to copper on the lichen photobiont Trebouxia ericiand the free-living algae Scenedesmus quadricauda[J].Plant Growth Regulation,2011,63(1):81-88.
- [8]Essaid L.Cd,Cr,Cu,Pb,and Zn concentrations in Ulva lactuca,Codium fragile,Jania rubens,and Dictyota dichoto-ma from Rabta Bay,Jijel(Algeria)[J].Environmental Monitoring and Assessment,2012,184(3):1711-1718.
- [9]Strezov A,Nonova T.Environmental monitoring of heavy metals in Bulgarian black sea green algae[J].Environmental Mo-nitoring and Assessment,2005(1/3),105:99-110.
- [10]Yoshida N,Ikeda R,Okuno T.Identification and characterization of heavy metal-resistant unicellular alga isolated fromsoil and its potential for phytoremediation[J].Bioresource Technology,2006,97(15):1843-1849.
- [11]Lu C M,Chan C W,Zhang J H.Acute toxicity of excess mercury on the photosynthetic performance of cyanobacterium,Splatensis:assessment by chlorophyll fluorescence analysis[J].Chemosphere,2000,41(1/2):191-196.
- [12]Zhang Y T,Fan X L,Yang Z M,et al.Characterization of H2 photoproduction by a new marine green alga,Platymonashelgolandica var tsingtaoensis[J].Applied Energy,2010,92:38-43.
- [13]赵娜,冯鸣凤,朱琳.不同pH值条件下Cr6+对小球藻和斜生栅藻的毒性效应[J].东南大学学报:医学版,2010,29(4):382-386.
- [14]温军杰,陈艳,胡勤海,等.螺旋藻对铅的耐受力初步研究[J].水资源保护,2005,21(3):24-27.
- [15]邱昌恩,况琪军,毕永红,等.Cd2+对绿球藻生长及生理特性的影响研究[J].水生生物学报,2007,31(1):142-145.
- [16]邱廷省,尹艳芬,蔡鲁晟.含锌重金属废水藻类吸附处理技术[J].水资源保护,2009,25(5):70-73.
- [17]Aksu Z,Donmez G.Binary biosorption of cadmium(Ⅱ)and nickel(Ⅱ)onto dried Chlorella vulgaris:co-ion effect onmonocomponent isotherm parameters[J].Process Biochemistry,2006,41(4):860-868.
- [18]Edding M,Tala F.Copper transfer and influence on a marine food chain[J].Bulletin of Environmental Contaminationand Toxicology,1996,57(4):617-624.
- [19]邱昌恩.六种常见重金属对藻类的毒性效应概述[J].重庆医科大学学报,2006,31(5):776-778.
- [20]Hammouda O,Gaber A,Abdelraouf N.Microalgae and wastewater treatment[J].Ecotoxicology and Environmental Safe-ty,1995,31(3):205-210.
- [21]Farhila M,Wan N N W N.Effects of metal ion concentrations on lipid and gamma linolenic acid production by Cunningha-mella sp.2A1[J].Online Journal of Biological Sciences,2008,8(3):62-67.
- [22]张传永,剖庆,陈燕妮.重金属对水生生物毒性作用研究进展[J].生命科学仪器,2008,11(6):3-7.
- [23]陈德辉,章宗涉,刘永定.微囊藻和栅藻共培养实验及其竞争参数的计算[J].生态学报,1999,19(6):908-913.
- [24]刘静,赵海涛,盛海军.Mn2+对四尾栅藻生长及Ca2+、Mg2+吸收的影响[J].水生态学杂志,2010,31(2):32-37.
- [25]Bligh E G,Dyer W J.A rapid method of total lipid extraction and purification[J].Canadian Journal of Biochemistryand Physiology,1959,37(8):911-917.
- [26]Monteiro C M,Castro P M L,Malcata F X.Cadmium removal by two strains of Desmodesmus pleiomorphus cells[J].Water,Air&Soil Pollution,2010,208(1/4):17-27.
- [27]Stabart A K,Griffiths W T,Sherwood R P,et al.The effect of Cd2+on the biosynthesis of chlorophyⅡin leaves of barley[J].Plant Physiol,1985,63(3):293-298.
- [28]袁敏,铁柏清,唐美珍.重金属单一污染对龙须草叶绿素含量和抗氧化酶系统的影响[J].土壤通报,2005,36(6):929-932.
- [29]Prassad D D K,Prassad A R K.Alteredδ-aminolaevulinic acid metabolism by lead and mercury in germinating seedlingsof Bajra(Pennisetum typhoideum)[J].Journal of Plant Physiol,1987,127(2):241-249.
- [30]Yan H,Pan G.Toxicity and bioaccumulati on of copper in three green microalgal species[J].Chemosphere,2002,49(5):471-476.
- [31]张怀成,孙景一,史致丽,等.重金属、螯合剂以及铁、锰离子对叉边金藻生长的影响及其混合效应[J].青岛海洋大学学报,1990,20(4):59-67.
- [32]Monteiro C M,Castro P M L,Malcata F X.Biosorption of zinc ions from aqueous solution by the microalga Scenedesmusobliquus[J].Environmental Chemistry Letters,2011,9(2):169-176.
- [33]孙珊,郑立,韩笑天,等.微藻油脂含量和组分及其影响因子的研究[J].海洋科学,2009,33(12):122-128.
- [34]Yeesang C,Cheirsilp B.Effect of nitrogen,salt,and iron content in the growth medium and light intensity on lipid pro-duction by microalgae isolated from freshwater sources in Thailand[J].Bioresource Technology,2011,102(3):3034-3040.
- [35]于殿宇,李默馨,孙博,等.脱色白土中重金属对大豆油脂品质影响的研究[J].食品工业科技,2011,32(10):107-113.