甘蔗UGPase cDNA的克隆及序列分析Cloning and Sequence Analysis of UDP-glucose Pyrophosphorylase cDNA from Saccharum officinarum
连玲;叶冰莹;何炜;张积森;何文锦;陈由强;陈如凯;
摘要(Abstract):
以甘蔗(FN95-1702)为材料,通过RT-PCR,首次克隆得到了甘蔗UGPase cDNA片段.该片段长1 495 bp,其中包含完整的ORF为1 431 bp,共编码476个氨基酸,并含有5个重要的Lys残基位点,分别为Lys257、Lys321、Lys367、Lys408、Lys409,它们对维持UGPase活性及与底物结合方面发挥着重要作用.
关键词(KeyWords): UGPase;基因克隆;序列分析
基金项目(Foundation): 国家“948”项目基金(2006-G37);; 农业公益性行业科研专项(nyhyzx07-019)
作者(Authors): 连玲;叶冰莹;何炜;张积森;何文锦;陈由强;陈如凯;
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