利用CRISPR/Cas9敲除小鼠低温诱导蛋白(CIRBP)基因Cold Induced RNA Binding Protein(CIRBP)Knockout Mice Using CRISPR/Cas9
范勇;肖楠阳;魏京京;许珊;罗倩萍;林清强;
摘要(Abstract):
为研究冷诱导RNA结合蛋白(CIRBP)与多种疾病的关系,利用CRISPR/Cas9系统敲除小鼠的CIRBP基因.针对CIRBP基因编码序列设计向导RNA(single guide RNA,sgRNA),构建相应sgRNA表达质粒.将此sgRNA与Cas9蛋白混合,注射C57BL/6小鼠的受精卵,实现靶基因敲除.通过T7E1酶切实验初步检测靶基因的修饰情况,再经过测序分析确定突变位点.获得了8个CIRBP基因突变的首建鼠,8只小鼠发生不同程度的碱基缺失.
关键词(KeyWords): 基因敲除;小鼠模型;CIRBP;CRISPR/Cas9
基金项目(Foundation):
作者(Authors): 范勇;肖楠阳;魏京京;许珊;罗倩萍;林清强;
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