范勇;肖楠阳;魏京京;许珊;罗倩萍;林清强;

• 1:福建师范大学南方生物医学研究中心
• 2:福建省天然免疫生物学重点实验室
• 3:福建师范大学生命科学学院

摘要(Abstract)：

为研究冷诱导RNA结合蛋白(CIRBP)与多种疾病的关系,利用CRISPR/Cas9系统敲除小鼠的CIRBP基因.针对CIRBP基因编码序列设计向导RNA(single guide RNA,sgRNA),构建相应sgRNA表达质粒.将此sgRNA与Cas9蛋白混合,注射C57BL/6小鼠的受精卵,实现靶基因敲除.通过T7E1酶切实验初步检测靶基因的修饰情况,再经过测序分析确定突变位点.获得了8个CIRBP基因突变的首建鼠,8只小鼠发生不同程度的碱基缺失.

关键词(KeyWords)： 基因敲除;小鼠模型;CIRBP;CRISPR/Cas9

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 范勇;肖楠阳;魏京京;许珊;罗倩萍;林清强;

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