曾邦定;梁玲;钟芳敏;王明兹;

• 1:福建师范大学工业微生物教育部工程研究中心
• 2:福建省麦丹生物集团有限公司福州研究中心

摘要(Abstract)：

以一株黄色短杆菌MDVR2-21为研究对象,利用响应面法对其发酵培养基进行优化,以提高L-缬氨酸产量.首先通过单因素实验确定了最适碳源为葡萄糖,最适无机氮源为硫酸铵,最佳有机氮源为黄豆饼粉和玉米浆.然后采用Plackett-Burman实验进行优化设计,筛选出影响L-缬氨酸生成的3个重要因素分别为葡萄糖、黄豆饼粉和硫酸铵.最后通过最陡爬坡实验及响应面中心组合设计获得最优发酵培养基配方为葡萄糖103.37 g·L^(-1)、黄豆饼粉23.96 g·L(-1)、黄豆饼粉23.96 g·L^(-1)、硫酸铵30.93 g·L(-1)、硫酸铵30.93 g·L^(-1)、玉米浆6 g·L(-1)、玉米浆6 g·L^(-1)、酵母膏2.4 g·L(-1)、酵母膏2.4 g·L^(-1)、Mg SO4·7H_2O 0.6 g·L(-1)、Mg SO4·7H_2O 0.6 g·L^(-1)、KH_2PO40.4 g·L(-1)、KH_2PO40.4 g·L^(-1)、Fe SO4·7H_2O 0.01 g·L(-1)、Fe SO4·7H_2O 0.01 g·L^(-1)、Mn SO4·3H_2O 0.01 g·L(-1)、Mn SO4·3H_2O 0.01 g·L^(-1)、Ca CO_335 g·L(-1)、Ca CO_335 g·L^(-1)、VB1100μg·L(-1)、VB1100μg·L^(-1)、VH 100μg·L(-1)、VH 100μg·L^(-1),p H 7.0.在优化培养基条件下摇瓶发酵48 h,L-缬氨酸产量达到33.2 g·L(-1),p H 7.0.在优化培养基条件下摇瓶发酵48 h,L-缬氨酸产量达到33.2 g·L^(-1),较优化前L-缬氨酸产量提高了10.32%.

关键词(KeyWords)： L-缬氨酸;黄色短杆菌;Plackett-Burman;响应面

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 曾邦定;梁玲;钟芳敏;王明兹;

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