苏经迁;刘辉英;郭凯;庄依凡;杨民和;

• 1:福建师范大学南方生物医学研究中心
• 2:福建师范大学生命科学学院
• 3:福建省天然免疫生物学重点实验室

摘要(Abstract)：

在7.0 L自动生物发酵罐中开展纤姿拟青霉(Paecilonmyces tennis)菌株YS-13的放大培养试验,并研究了不同培养阶段的各项发酵参数的变化情况.结果表明:P.tennis YS-13在发酵罐扩大培养条件下石杉碱甲的产量达到20.0μg·mL^(-1),其中待测组分B5-8对乙酰胆碱酯酶的半数抑制浓度(ρIC50)为2.38mg·mL(-1),其中待测组分B5-8对乙酰胆碱酯酶的半数抑制浓度(ρIC50)为2.38mg·mL^(-1);同时获得了P.tennis YS-13在发酵罐培养过程中p H值、菌体质量浓度、产物浓度等参数的变化情况,为今后的工艺优化奠定了基础.

关键词(KeyWords)： 自动生物发酵罐;石杉碱甲;纤姿拟青霉(Paecilonmyces tennis YS-13);扩大培养

Abstract:

Keywords:

基金项目(Foundation): 福建省科技厅重点项目(2012N0013);; 福建省中青年教师教育科研项目(JAT160107)

作者(Authors): 苏经迁;刘辉英;郭凯;庄依凡;杨民和;

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