魏文杰;邓霞;杨淑慎;

• 1:西北农林科技大学生命科学学院

摘要(Abstract)：

探究质体形式的GAPCp (Plastidial glyceraldehyde-3-phosphate dehydrogenase)基因启动子响应干旱、盐和外源ABA等非生物胁迫的机理.首先从中国春小麦中克隆得到TaGAPCp1基因上游1 985 bp的核苷酸序列,经Plant CARE数据库分析表明,该启动子含有众多防御和逆境响应元件(defense and stress-responsive ele-ments,DSREs),激素响应元件(hormone-responsive elements,HREs)和光响应元件(light-responsive ele-ments,LREs).从小麦数据库下载Ta GAPCp亚家族基因启动子序列,序列对比分析表明,该亚家族成员启动子上功能元件种类相近但数量相差较大.构建含TaGAPCp1基因启动子的表达载体,通过农杆菌介导法转化烟草并进行ABA (100μmol·L^(-1))、PEG8000 (20%)、Na Cl (250 mmol·L(-1))、PEG8000 (20%)、Na Cl (250 mmol·L^(-1))和4℃低温处理,组织化学染色显示TaGAPCp1基因启动子能驱动GUS基因的表达但活性明显低于Ca MV35S,GUS活性分析显示TaGAPCp1基因启动子能响应非生物胁迫.通过农杆菌介导的浸花法转化拟南芥,经潮霉素和羧苄青霉素筛选及叶片PCR检测,最终获得稳定遗传的转基因拟南芥20株,并得知转基因拟南芥中的GUS基因能被干旱胁迫显著诱导表达.

关键词(KeyWords)： 小麦;TaGAPCp1基因启动子;逆境胁迫;稳定表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(31271625、31271609);; 黄土高原土壤侵蚀与旱地农业国家重点实验室专项(10502)

作者(Authors): 魏文杰;邓霞;杨淑慎;

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