ScINO1基因克隆及酵母多基因多拷贝整合表达载体的构建Cloning of ScINO1 Gene and Construction of Yeast Multi-copy Integration Expression Vector
黄贞杰;陈玲;张积森;叶冰莹;陈由强;陈如凯;
摘要(Abstract):
利用PCR技术,以酿酒酵母S288c的基因组DNA为模板,扩增酿酒酵母肌醇-1-磷酸合成酶基因(ScINO1)包含1 602bp的开放读码框,克隆后经PCR和酶切反应进行了鉴定,测序结果与GenBank上已登录的ScINO1序列完全一致.对PUG6载体进行改造,构建了rDNA介导的多拷贝整合表达载体pURH,并将ScINO1基因连入载体pURH,获得ScINO1基因超表达载体pURIH.为下一步的微生物发酵法产肌醇和获得能够耐高乙醇的酵母工程菌株的研究奠定基础.
关键词(KeyWords): ScINO1基因;多拷贝整合;载体构建;酿酒酵母
基金项目(Foundation): 现代农业产业技术体系建设专项资金资助项目(CARS-20-4-4)
作者(Authors): 黄贞杰;陈玲;张积森;叶冰莹;陈由强;陈如凯;
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