苏云金芽孢杆菌aiiA基因启动子的克隆及功能分析Cloning and Functional Verification of Promoter of aiiA Gene from Bacillus thuringiensis
温真;杨彩云;林丽玉;毛惠民;杨梅;
摘要(Abstract):
根据已报道的aiiA基因序列(AY460124)设计特异性PCR引物,扩增得到aiiA基因启动子序列的单一产物.将该启动子序列代替pET-28a-GFP质粒上的T7启动子,构建绿色荧光蛋白功能分析载体.经测序比对和生物信息学软件分析发现该序列含有6个可能的启动区域和19个不同类型的转录结合位点.最后通过荧光显微镜观察到绿色荧光蛋白发出荧光,从而分析了该序列的启动子活性.
关键词(KeyWords): 苏云金芽胞杆菌;启动子;绿色荧光蛋白;功能鉴定
基金项目(Foundation): 福建省科技厅资助项目(2009N0032);; 福建省教育厅资助项目(JA08041)
作者(Authors): 温真;杨彩云;林丽玉;毛惠民;杨梅;
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