甘蔗果糖-6-磷酸,2-激酶/果糖-2,6-二磷酸酶基因的cDNA克隆及序列分析Cloning and Sequence Analysis of Fructose-6-Phosphate,2-Kinase/Fructose-2,6-Bisphosphatase from Saccharum officenarum
何炜;叶冰莹;周平;郑钊;张积森;何文锦;林荣华;陈由强;陈如凯;
摘要(Abstract):
以高等植物玉米、水稻的果糖-6-磷酸,2-激酶/果糖-2,6-二磷酸酶(F2KP)的保守区域设计引物,并采用逆转录——聚合酶链式反应(RT-PCR)方法,从甘蔗叶片中扩增F2KP的cDNA片段,命名为SoF2KP-L.该cDNA片段长2 178 bp,含2 085 bp的完整开放阅读框,编码694个氨基酸.序列分析表明,甘蔗F2KP与其它已知的高等植物F2KP具有很高的同源性.
关键词(KeyWords): 甘蔗;果糖-6-磷酸,2-激酶/果糖-2,6-二磷酸酶;基因克隆;序列分析
基金项目(Foundation): 国家“948”项目基金(2006-G37);; 农业公益性行业科研专项(nyhyzx07-019)
作者(Authors): 何炜;叶冰莹;周平;郑钊;张积森;何文锦;林荣华;陈由强;陈如凯;
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