苏云金芽孢杆菌aiiA基因的克隆与结构分析Cloning and Analysis of aiiA Gene from Bacillus thuringiensis
杨梅;林彬辉;关夏玉;郭丽清;姚帆;黄天培;黄志鹏;关雄;
摘要(Abstract)：
分别克隆了叶片、苔藓和土壤中分离的Bacillus thuringiensis的aiiA基因,并对其编码的蛋白AiiA-P28、AiiA-B19和AiiA-S2进行了理化特征分析、进化树分析和空间结构的研究.结果表明AiiA-P28、AiiA-B19和AiiA-S2分子质量均为28 ku;等电点分别为4.77、4.77、4.93;三者均为亲水性蛋白,具有很高的同源性.进化树分析结果表明叶片中分离的AiiA-P28与苔藓中分离的AiiA-B19进化距离较近,而土壤中分离的AiiA-S2则与它们的进化距离较远.三维结构分析表明,AiiA蛋白具有典型的αβ/βα折叠的空间结构.
关键词(KeyWords)： 苏云金芽孢杆菌;aiiA基因;进化树分析;空间结构
基金项目(Foundation): 福建省科技厅基金资助项目(2005K019Z05160072006J0051)
作者(Author): 杨梅;林彬辉;关夏玉;郭丽清;姚帆;黄天培;黄志鹏;关雄;
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