应用草履虫建立中药快速毒性筛选实验方法的探讨Application of Paramecium caudatum to Set up Acute Toxicity Study on Traditional Chinese Medicine
李钻芳;陈文列;曾红;蒋定桦;刘安娜;林如辉;黄美雅;黄云梅;
摘要(Abstract):
应用草履虫进行中药快速毒性实验,拟建立药物的单细胞动物快速毒性筛选实验的基本方法.分别进行慢性与急性毒性实验观察草履虫的应激性、运动状态、存活只数.结果显示,在慢性实验的48 h内,草履虫的存活数量与时间、质量浓度呈指数递增.当中药质量浓度为5 mg·mL(-1)时,草履虫开始出现死亡.急性毒性实验6 h内,药物低质量浓度组未见草履虫死亡.而中、高质量浓度组,随着药物质量浓度、时间增加,毒性也增加.上述研究结果表明,急性毒性实验可快速获得药物安全剂量、最大耐受剂量、致死剂量、半数致死量等参数,可快速进行药物毒性评价和毒性药物筛选.
关键词(KeyWords): 草履虫;单细胞动物;中药;急性毒性实验
基金项目(Foundation): 福建省自然科学基金资助项目(2014J01356);; 福建省教育厅资助项目(JA11131)
作者(Authors): 李钻芳;陈文列;曾红;蒋定桦;刘安娜;林如辉;黄美雅;黄云梅;
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