沈秀球;黄义梅;杨洪钦;郑莉琴;王瑜华;谢树森;

• 1:医学光电科学与技术教育部重点实验室福建师范大学激光与光电子技术研究所

摘要(Abstract)：

成功实现体外培养背根神经元(Dorsal Root Ganglion,DRG),利用差速贴壁法进行提纯,通过细胞免疫荧光技术鉴定所培养的细胞纯度,发现其纯度高达90%,完全适合运用于细胞分子机理研究.在此基础上,采用激光共聚焦显微成像实验观察DRG内的Ca2+荧光信号,并对Ca2+浓度进行了定量分析.研究结果有助于深入探究和定量分析神经元中Ca2+参与特定的细胞生理功能.

关键词(KeyWords)： 背根神经节(DRG);神经元;疼痛;Ca2+;fluo-3/AM

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(60978071);; 福建省自然科学基金资助项目(2010J01322)

作者(Authors): 沈秀球;黄义梅;杨洪钦;郑莉琴;王瑜华;谢树森;

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