邱丹缨;温扬敏;郑晨娜;罗彩林;蔡炼;

• 1:泉州医学高等专科学校基础医学部
• 2:泉州市医药研究所

摘要(Abstract)：

为从红树林微生物中分离抗肿瘤活性物质,从泉州湾红树土壤中分离、纯化和筛选能产生抗肿瘤活性物质的细菌,并利用16S rDNA序列比对方法进行分子鉴定.采用追踪分离的方式,以稻瘟霉分生孢子抑制强度和肿瘤细胞体外抑制活性为指标,对细菌发酵液抗肿瘤活性成分进行初步分离.实验结果显示,从泉州湾红树林土壤中分离出1株具有较强抗肿瘤活性的细菌,经鉴定为Vibrio fluvialis. Vibrio fluvialis发酵液能抑制稻瘟霉分生孢子萌发时菌丝形变,并抑制EC9706食管癌细胞和H22肝癌细胞生长.从Vibrio fluvialis发酵液分离出具有较强抗肿瘤活性的化学组分Ⅰ和组分Ⅱ.组分Ⅰ对EC9706食管癌细胞和H22肝癌细胞ρIC50分别为0. 18 mg·m L^(-1)和0. 26 mg·m L(-1)和0. 26 mg·m L^(-1),组分Ⅱ对EC9706食管癌细胞和H22肝癌细胞ρIC50分别为0. 17 mg·m L(-1),组分Ⅱ对EC9706食管癌细胞和H22肝癌细胞ρIC50分别为0. 17 mg·m L^(-1)和0. 22 mg·m L(-1)和0. 22 mg·m L^(-1).表明Vibrio fluvialis能产生抗肿瘤活性物质,具有重要抗肿瘤研发价值.

关键词(KeyWords)： 抗肿瘤;细菌;活性物质;分离

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金资助项目(2016J01420);; 泉州市高端海洋人才引进资助项目(泉委人才[2016] 2号);; 泉州市科技计划项目(2015Z92)

作者(Authors): 邱丹缨;温扬敏;郑晨娜;罗彩林;蔡炼;

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