杨梅;林丽玉;温真;杨彩云;

• 1:福建师范大学生命科学学院

摘要(Abstract)：

通过对工程菌E.coli BL21(DE3)-pET29a-aiiA的摇瓶发酵培养条件进行优化,确定了AiiA蛋白可溶性表达的最佳发酵培养基为牛肉膏蛋白胨.目的蛋白可溶性表达的最佳诱导条件为:IPTG浓度0.6 mmol/L,诱导初始OD600为0.5,诱导时间为20 h,温度20℃,pH为7.0,微量元素Na2HPO420 mmol/L,250 mL三角瓶的装液量为70 mL.优化前可溶性AiiA蛋白的相对含量为2.23 mg/mL,占总目的蛋白的31.4%,优化后达到4.54 mg/mL,占总目的蛋白的60.3%,大大提高了目的蛋白的可溶性表达.

关键词(KeyWords)： AiiA蛋白;发酵;条件优化

Abstract:

Keywords:

基金项目(Foundation): 福建省科技厅资助项目(2009N0032);; 福建省教育厅资助项目(JA08041)

作者(Authors): 杨梅;林丽玉;温真;杨彩云;

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