江剑平;江方璐;李幼萍;

• 1:福建师范大学生命科学学院
• 2:北京中医药大学东方学院

摘要(Abstract)：

探讨应用腹水传代培养的Walker256大鼠乳腺癌细胞系建立SD大鼠胫骨癌痛模型,为骨癌痛的研究和镇痛药物开发创造有利条件.结果显示,在骨癌模型组,接种后体质量呈缓慢下降,从第15天起与接种前相比存在极显著差异,到第21天时,体质量下降了12. 5%(P <0. 001);热缩足潜伏期也逐渐下降,从第6天开始,与接种前相比存在显著性差异,至第21天时,缩足潜伏期下降了34. 1%,接种的对侧后足也出现类似现象; X-影像观察显示骨癌痛模型大鼠的胫骨结构受到破坏,骨密度不均一.以上结果表明,应用腹水传代的Walker256乳腺癌细胞接种后能产生较为稳定的痛觉过敏等骨癌痛行为,SD大鼠胫骨癌痛模型建立成功.

关键词(KeyWords)： Walker256乳腺癌细胞;腹水传代;骨癌痛模型;热缩足潜伏期

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(31371124);; 福建省自然科学基金资助项目(2014J01305);; 福建师范大学生命科学学院“溪源江学者”扶持计划(FZSKG2018005)

作者(Authors): 江剑平;江方璐;李幼萍;

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