毛惠民;林丽玉;杨彩云;温真;曾世涌;杨梅;

• 1:福建师范大学生命科学学院

摘要(Abstract)：

根据GenBank中aiiA基因设计引物,以苏云金芽孢杆菌基因组为模板扩增出aiiA基因后构建出pPIC9K-aiiA重组表达载体,线性化后转化毕赤酵母GS115,获得重组工程菌GS115/pPIC9K-aiiA,以体积分数为1%的甲醇进行诱导表达.表达产物经SDS-PAGE及Western blotting分析显示表达的蛋白具有较好的免疫特异性.抗病性实验显示表达的目的蛋白具有生物学活性和良好的抗病能力.

关键词(KeyWords)： GS115;aiiA基因;pPIC9K;分泌表达

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金资助项目(2012J01123)

作者(Authors): 毛惠民;林丽玉;杨彩云;温真;曾世涌;杨梅;

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