响应面法优化蓝莓花青素提取工艺参数Optimization of the Extraction Process Parameters of Blueberry Anthocyanin by Response Surface Method
薛婷;原雪;冯佳乐;林荣华;陈由强;
摘要(Abstract)：
为研究从蓝莓中提取花青素的最佳方法,采用微波-乙醇法处理原料蓝莓皮.在单因素设计的基础上,选取提取时间、微波功率、乙醇体积分数为自变量,以蓝莓花青素提取率为响应值,运用响应面法研究各自变量及其交互作用对蓝莓花青素提取率的影响.结果表明:微波-乙醇法提取蓝莓花青素的最佳工艺条件:提取时间4.15 min,乙醇体积分数65.8%,微波功率440 W,料液比为1∶20,在此条件下花青素提取率为87.07%,与预测的相对误差小,回归模型拟合情况较好,预测能力良好.
关键词(KeyWords)： 蓝莓;花青素;微波-乙醇;响应面;提取率
基金项目(Foundation): 农业部现代农业产业技术体系建设专项资金(CARS-20-4-4)
作者(Authors): 薛婷;原雪;冯佳乐;林荣华;陈由强;
参考文献(References)：
- [1]陈卫.蓝莓及其营养保健功能[J].中外食品,2003,7:34-35.
- [2]顾姻,贺美安.蓝浆果与蔓越橘[M].北京:中国农业出版社,2001.
- [3]CHEN J,TAO X,ZHANG M,et al.Properties and stability of blueberry anthocyanin-bovine serum albumin nanoparticles[J].Journal of the Science of Food and Agriculture,2014,94(9):1781-1786.
- [4]MENG X,YIN B,FENG H L,et al.Overexpression of R2R3-MYB gene leads to accumulation of anthocyanin and enhanced resistance to chilling and oxidative stress[J].Biologia Plantarum,2014,58(1):121-130.
- [5]LIANG Y,CHEN J,ZUO Y,et al.Blueberry anthocyanins at doses of 0.5%and 1%lowered plasma cholesterol by increasing fecal excretion of acidic and neutral sterols in hamsters fed a cholesterol-enriched diet[J].Eur J Nutr,2013,52(3):869-875.
- [6]LI Q S,XIAO P D,JIN Q,et al.Antioxidant anthocyanins screening through spectrum–effect relationships and DPPHHPLC-DAD analysis on nine cultivars of introduced rabbiteye blueberry in China[J].Food Chemistry,2012,132(2):759-765.
- [7]ZU X Y,ZHANG Z Y,ZHANG X W,et al.Anthocyanins extracted from Chinese Blueberry(Vaccinium uliginosum L.)and its anticancer effects on DLD-1 and COLO205 cells[J].Chin Med J,2010,123(19):2714-2719.
- [8]ZHANG Y J,HU Z L,CHU G H,et al.Anthocyanin accumulation and molecular analysis of anthocyanin BiosynthesisAssociated genes in Eggplant(Solanum melongena L.)[J].Journal of Agricultural and Food Chemistry,2014,62(13):2906-2912.
- [9]孙磊.乙醇提取苦瓜皂甙的工艺研究[J].宁夏医学杂志,2005,27(2):112-114.
- [10]陈晓平,于翠翠.响应面法优化微波辅助乙醇提取桑黄黄酮工艺的研究[J].食品与发酵科技,2013(4):31-36.
- [11]赵子铭,付刚,李诚.响应面法微波辅助乙醇提取红景天苷工艺优化[J].食品科技,2013(14):147-151.
- [12]李耀玲,林启训,庞杰.微波辅助乙醇提取决明子黄酮的工艺优化[J].莆田学院学报,2013(2):41-45.
- [13]何肖,王文凯,马明波,等.香草醛盐酸法定量薯莨色素提取液中的缩合单宁[J].现代纺织技术,2014(6).
- [14]李春阳,许时婴,王璋.香草醛-盐酸法测定葡萄籽、梗中原花青素含量的研究[J].食品科学,2004,2:157-161.
- [15]李春阳,许时婴,王璋.低浓度香草醛-盐酸法测定葡萄籽、梗中原花青素含量的研究[J].食品工业科技,2004,6:128-130.
- [16]陈珊珊,丁俏羽,李佳,等.响应面法优化虾壳可溶性钙提取工艺[J].中国食品学报,2014,14(6):81-89.
- [17]肖丽霞,何志贵,朱勇,等.葡萄籽中原花青素提取工艺[J].食品科学,2011,32(20):89-94.
- [18]王竹清,王军.“赤霞珠”葡萄皮渣中原花青索的提取工艺研究[J].食品科学,2009,30(12):101-106.