黄义德;林玮;蔡燕惠;张彦定;

• 1:福建农林大学生命科学学院
• 2:福建师范大学生命科学学院
• 3:福建师范大学生命科学学院
• 4:福建师范大学生命科学学院 福建福州350002

摘要(Abstract)：

试图建立人骨形态发生蛋白-4在毕赤酵母(Pichia pastoris)中的表达技术.包括:将hBMP4成熟片段从全长cDNA亚克隆到分泌型表达载体质粒pPIC9K多克隆位点上,构建能表达rhBMP4的载体质粒pPIC9K-hBmp4.经转化Pichia pastoris宿主菌株GS115,PCR鉴定基因整合后,摇瓶培养,用甲醇诱导表达.表达上清液经SDS-PAGE和Western-blot分析,结果表明rhBMP4以单体形式分泌到发酵上清液中,单体分子质量大小约为26 ku,摇瓶表达量达到20.13μg/mL.

关键词(KeyWords)： 人骨形态发生蛋白-4;毕赤酵母;重组表达

Abstract:

Keywords:

基金项目(Foundation): 福建省教育厅重点资助项目(JA01015);; 福建省发改委基金资助项目(省发改[2004686号]);; 福建省自然科学基金资助项目(C0410008)

作者(Authors): 黄义德;林玮;蔡燕惠;张彦定;

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