黄义德;吴金芸;徐轶彦;韩明;林政;

• 1:福建师范大学生命科学学院
• 2:福建省发育与神经生物学重点实验室
• 3:福建卫生职业技术学院

摘要(Abstract)：

为了了解在毕赤酵母中人骨形态发生蛋白4基因剂量对其表达量的影响,利用毕赤酵母表达载体pAO815质粒上的2个同尾酶BglⅡ和BamHⅠ在体外构建了人骨形态发生蛋白4成熟肽序列1,3,6,9个拷贝表达盒,并对它们进行表达量研究.结果表明,在毕赤酵母中表达人骨形态发生蛋白4蛋白时,1个拷贝基因能达到最大表达量,增加人骨形态发生蛋白4基因的拷贝数并不会增加人骨形态发生蛋白4的表达量,基因拷贝数的增加反而导致人骨形态发生蛋白4在毕赤酵母中的表达水平下降.

关键词(KeyWords)： 毕赤酵母;人骨形态发生蛋白4;基因拷贝数

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金资助项目(2009J01127);; 福建省教育厅资助项目(JB09038)

作者(Authors): 黄义德;吴金芸;徐轶彦;韩明;林政;

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