伊魁宇;韩永和;许尊炼;王猛;

• 1:中国刑事警察学院法化学系
• 2:福建师范大学生命科学学院
• 3:中国刑事警察学院痕迹检验技术系

摘要(Abstract)：

以巯基乙酸为稳定剂在水相中合成了尺寸均匀的CdTe量子点(QDs).考查了磷酸盐(PBS)缓冲体系中量子点与牛血红蛋白(Bovine Hemoglobin,BHb)之间的作用规律.结果表明,巯基乙酸修饰的CdTe量子点与BHb有较强的作用.BHb能使修饰后的CdTe量子点发生荧光淬灭现象,并且BHb的浓度以及酸度对荧光淬灭均有影响,并探讨了导致荧光淬灭的原因以及量子点与血红蛋白相互作用的机理.

关键词(KeyWords)： CdTe量子点;牛血红蛋白;荧光淬灭

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(21205139);; 公安部应用创新计划项目(2012YYCXXJXY127);; 中国刑事警察学院大学生创新训练计划项目(201210175033)

作者(Authors): 伊魁宇;韩永和;许尊炼;王猛;

参考文献(References)：

• [1]Alivisatos A P.Perspectives on the physical chemistry of semiconductor nanocrystals[J].J Phys Chem,1996,100(31):13226-13239.
• [2]Peng X G,Manna L,Yang W D,et al.Shape control of CdSe nanocrystals[J].Nature,2000,404(6773):59-61.
• [3]Weller H.Colloidal semiconductor Q-particles:chemistry in the transition region between solid state and molecules[J].Angew Chem Int Ed,1993,32(1):41-53.
• [4]Wang Y,Herron N.Nanometer-sized semiconductor clusters:materials synthesis,quantum size effects,and photophysi-cal properties[J].J Phys Chem,1991,95(2):525-532.
• [5]Mattoussi H,Mauro J M,Bawendi M G.Self-assembly of CdSe-ZnS quantum dot bioconjugates using an engineered re-combinant protein[J].J Am Chem Soc,2000,122(49):12142-12149.
• [6]Mitchell G P,Mirkin C A,Letsinger R L.Programmed assembly of DNA functionalized quantum dots[J].J Am Chem Soc,1999,121(35):8122-8123.
• [7]Cao Q E,Wang K,Hu Z,et al.Syntheses of three new derivatives of8-aminoquinoline and its applications for fluorimet-ric determination of copper(II)[J].Talanta,1998,47(4):921-927.
• [8]董再蒸,周华萌,徐淑坤,等.CdTe量子点酸度敏感荧光探针测定水样中铵根离子含量[J].分析化学,2009,37(8):1215-1218.
• [9]Li M Y,Zhou H M,Zhang H Y,et al.Preparation and purification of L-cysteine capped CdTe quantum dots and its self-recovery of degenerate fluorescence[J].J Lumin,2010,130(10):1935-1940.
• [10]Wang L,Chen H Q,Wang L Y,et al.Preparation and application of a novel composite nanoparticle as a protein fluo-rescence probe[J].Anal Lett,2004,37(2):213-223.
• [11]Chen X D,Wang X B,Liu L,et al.Functionalized semiconductor nanocrystals for ultrasensitive detection of peptides[J].Anal Chim Acta,2005,542:144–150.
• [12]Liang J G,Huang S,Zeng D Y,et al.CdSe quantum dots as luminescent probes for spironolactone determination[J].Talanta,2006,69:126–130.
• [13]Ma Y,Yang C,Li N,et al.A sensitive method for the detection of catecholamine based on fluorescence quenching of CdSe nanocrystals[J].Talanta,2005,67:979–983.
• [14]Dong C Q,Qian H F,Fang N H,et al.Study of fluorescence quenching and dialysis process of CdTe quantum dots,u-sing ensemble techniques and fluorescence correlation spectroscopy[J].J Phys Chem B,2006,110(23):11069-11075.
• [15]Ferna'ndez-Arguelles M T,Wei J J,Costa-Ferna'ndez J M,et al.Surface-modified CdSe quantum dots for the sensitive and selective determination of Cu(Ⅱ)in aqueous solutions by luminescent measurements[J].Anal Chim Acta,2005,549:20–25.
• [16]Liang J G,Ai X P,He Z K,et al.Functionalized CdSe quantum dots as selective silver ion chemodosimeter[J].An-alyst,2004,129:619–622.
• [17]Gatt'as-Asfura K M,Leblanc R M.Peptide-coated CdS quantum dots for the optical detection of copper(Ⅱ)and silver(Ⅰ)[J].Chem Commun,2003:2684-2685.
• [18]Wei J J,Costa Fern'andez J M,Pereiro R,et al.Surface-modified CdSe quantum dots as luminescent probes for cya-nide determination[J].Anal Chim Acta,2004,522:1-8.
• [19]Huang C P,Li Y K,Chen T M.A highly sensitive system for urea detection by using CdSe/ZnS core-shell quantum dots[J].Biosens Bioelectron,2007,22(8):1835-1838.
• [20]Deng Z T,Zhang Y,Yue J C,et al.Green and orange CdTe quantum dots as effective pH-sensitive fluorescent probes for dual simultaneous and independent detection of viruses[J].J Phys Chem B,2007,111(41):12024-12031.
• [21]Yu D H,Wang Z,Liu Y,et al.Quantum dot-based pH probe for quick study of enzyme reaction kinetics[J].En-zyme Microb Tech,2007,41(1/2):127-132.
• [22]张雨琴,张友玉,叶敏,等.光度法研究L-半胱氨酸修饰的ZnS纳米粒子与牛血红蛋白的作用[J].应用化学,2008,25(9):1011-1016.
• [23]韦静,沈星灿,梁宏,等.牛血红蛋白与纳米雄黄相互作用的光谱研究[J].光谱学与光谱分析,2008,28(4):852-855.
• [24]Li M Y,Ge Y X,Chen Q F,et al.Hydrothermal synthesis of highly luminescent CdTe quantum dots by adjusting pre-cursors’concentration and their conjunction with BSA as biological fluorescent probes[J].Talanta,2007,72(1):89-94.
• [25]Yu W W,Qu L H,Guo W Z,et al.Experimental determination of the extinction coefficient of CdTe,CdSe,and CdS nanocrystals[J].Chem Mater,2003,15(14):2854-2860.